Construction and characterization of murine single-chain variable fragment (MuscFv) antibody against acrylamide in coffee

Sukanya Ponphimai, Parichat Srinok, Nopporn Naewwan, Thitimakorn Namhong, Jeeraphong Thonongsaksrikul, Sanong Suksaweang, Theeraya Simawaranon and Kanyarat Thueng-In*

Ingredients of food, especially sugar and starch at high-temperature cooking processes could lead to the formation of acrylamide (AA). This chemical is a harmful carcinogen, a neurotoxicant, a reproductive toxicant, and a carcinogen in animal species. However, the detection of acrylamide contamination in food goes unnoticed. In this work, the mouse monoclonal antibody in the form of a single chain variable fragment (scFv) specific to acrylamide was selected from the murine scfv (muscfv) phage-displayed library. Acrylamide (AA) was used as an antigen for bio-panning. The murine single-chain variable fragment (MuscFv) antibody specific to acrylamide in coffee was successfully constructed, which was determined by ELISA and HPLC. Currently, this is the first study, which describes the selection of antibodies against acrylamide from the muscfv phage-displayed library and could be used as a tool for the detection of acrylamide in coffee.

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Published on: May 28, 2024 Pages: 9-16

Full Text PDF Full Text HTML DOI: 10.17352/abse.000032
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